Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 17 Issue 1
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- Pages.69-73
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Some Properties of Clostridium butyricum ID-113 Autolytic enzyme
Clostridium butyricum ID의 자가분해 효소
- Kwag, Jong-Hui (Department of Microbiology, Research Labortories, Il-Dong Pharm. Co., Ltd.) ;
- Lee, Se-Yong (Department of Agricultural Chemistry, Korea University) ;
- Kim, Tre-Han (Department of Microbiology, Research Labortories, Il-Dong Pharm. Co., Ltd.) ;
- Lee, Jung-Chi (Department of Microbiology, Research Labortories, Il-Dong Pharm. Co., Ltd.)
- Published : 1989.02.01
Abstract
Cellular autolytic enzyme was isolated from the supernatant fluid of exponentially growing cuiture of Cl. butyricum ID-113. The autolysin was partially pruified by ammonium sulfate fractionation, chromatography on DEAE-Sephadex A-50 and gel filtration through Sephadex G-200. This autolytic enzyme lysed SDS-treated cell wall fractions of Cl. butyricum ID, but not whole cells at all. Its optimum pH and temperature were 5.0 and 37
자가분해 효소는 Cl. butyricum의 포자형성 배지에서 배양할 때 체외로 배출되어 배양액에도 존재하였다. 배양액으로부터 약 50배로 부분정제된 자가분해 효소를 사용하여 효소의 성질을 조사하였다. 자가분해 효소의 최적 pH와 온도는 각각 5.0과 37