Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Intracellular Accumulation of Cadmium by Intact Cadmium Tolerant Yeast Cells

카드뮴 내성 효모의 Intact Cells에 의한 카드뮴의 세포내 축적

  • Yu, Tae-Shick (Department of Biology, College of Natural Science, Keimyung University) ;
  • Song, Hyung-Ik (Department of Food Technology, College of Agriculture, Kyungpook National University) ;
  • Chung, Ki-Taek (Department of Food Technology, College of Agriculture, Kyungpook National University)
  • Published : 1989.02.01
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Abstract

An intracellular accumulation of cadmium by the intact cell of an extremely cadmium tolerant yeast, Hansenula anomala B-7, was investigated in the presence of Triton X-100. The uptake of cadmium by the intact cell was efficiently enhanced up to approximately 40% or more by 0.1% of Triton X-100 and Aerosol OT, respectively. The Michaelis constant, Km, done by Lineweaver-Burk plot of accumulation velocity of cadmium vs. cadmium concentration was calculated to be 0.247mM. The optimal conditions of pH and the temperature for the effective cadmium uptake were from neutrality to alkali and 4$0^{\circ}C$, respectively. The accumulation of cadmium was increased approximately 3 times under the shaking incubation, with no correlation to shaking rate. By zinc the cadmium accumulation was decreased.

고도 카드뮴 내성 효모, Hansenula anomala B-7 의 intact cell에 의한 카드뮴의 세포내 축적에 미치는 계면활성제의 영향를 검토하고, Triton X-100의 존재하에서의 카드뮴 축적에 미치는 제인자 등을 검토했다. 카드뮴의 축적은 0.1% Triton X-100과 Aerosol OT에 의하여 약 40% 이상 증가되었다. 카드뮴 이온에 대한 Km값은 0.247mM로 계산되었다. 카드뮴의 축적 최적 pH는 pH7.0에서 pH10.0 사이였으며, 최적온도는 4$0^{\circ}C$였다. 카드뮴의 최적 축적은 정치보다 진탕하므로 약 3배 증가되었으며, 진탕속도는 영향을 미치지 않았다. 카드뮴과 아연 이온을 공존시키므로 카드뮴의 축적이 아연 이온에 의하여 저해되었다.

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