Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 17 Issue 1
- /
- Pages.14-18
- /
- 1989
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Ethanol Fermentation of Fusant between Heterologous Transformant of Saccharomyces cerevisiae and Candida tropicalis in Pilot Scale
Pilot Scale에서의 Fusant의 Ethanol 발효
- Seu, Jung-Hwn (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
- Kim, Young-Ho (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
- Lee, Soon-Mo (Laboratory, Phung Gook Alcohol Industry Co.) ;
- Bang, Byung-Ho (Department of Food Nutrition, Seoul Health Junior College)
- Published : 1989.02.01
Abstract
As the final experiment to assess the possibility of industrial application of FSC14-75, ethanol productivity from liquefied sweet potato starch was examined in a pilot scale of 300 liters. FSC14-75 produced 6.6%(v/v) of ethanol from 13.3% of liquefied sweet potato starch in 8 days, and the residual sugar was 3.15%. The corresponding efficiency was 70% of the theoretical maximum. Since we could isolate unicellular cell and flocculent cell from the fermentation broth, we designated them FSC14-75(S) and FSC14-75(F), respectively. We investigated ethanol productivity of FSC14-75(F) compared with that of FSC14-75(S) from liquefied potato starch in a mini·tar tormentor scale of 2.5 liters. FSC14-75(F) was found more favorable than the counterpart in terms of ethanol productivity, and produced 8.1%(v/v) of ethanol from 15% of liquefied potato starch with an efficiency of 75%. In a pilot scale fermentation with 15% of liquefied sweet potato starch, ethanol productivity of FSC14-75(F) reached maximum level of 7.7%(v/v) after 8 days, and the residual sugar was 1.9%. However, the ethanol productivity was not enhanced by a supplementary addition of Thermamyl to the fermentation broth after sterilization.
Fusant FSC14-75의 산업적 이용가능성을 검토하기 위한 최종 실험으로서 Working volume이 300 liters인 pilot scale에서의 발효력을 조사하였다. 13,3%의 liquefied sweet potato starch (Total sugar=14.8%)을 ethanol 발효원으로 함유한 fermentation broth 300liters를 0.6% seed로 발효시킨 결과 발효 최종일인 8일만에 6.6%(v/v)의 ethanol을 생성하였고 이때 잔당은 15%이었으므로 발효율은 총당에 대해서는 70%, 소비당에 대해서는 87.5%로 나타났다. 한편 fermentation broth로부터 FSC-14-75 유래의 unicellular cell과 flocculent cell을 분리할 수 있었으며 이를 각각 FSC14-75(S)와 FSC14-75(F)로 명명하였고, 이들 두 균주의 ethanol 발효능을 mini-jar fermentor scale에서 조사한 결과 FSC14-75(F)가 ethanol productivity에 있어서 우수하였으며 15%의 liquefied potato starch로부터 발효 10일만에 8.1%(v/v)의 ethanol을 생성하여 발효율은 총당에 대해 75%였다. FSC-14-73 (F)의 ethanol productivity를 pilot scale에서 조사하기 위해 총당 15.33%의 liquefied sweet potato starch를 함유한 fermentation broth 300 liters를 1% seed로써 발효시킨 결과 ethanol 생성은 배양 8일만에 최대 7.7%(v/v)였으며 이때 잔당은 1.9%였다. 또한 FSC-14-75(F) 균주의 발효과정중