KSBB Journal
- Volume 4 Issue 1
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- Pages.21-30
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- 1989
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
Alcohol Production by Extractive Fermentation in a Continuous Bioreactor
연속 생물반응기 안에서 유출 발효에 의한 알코올 생산
Abstract
Lauryl alcohol was used as extracting solvent of ethanol, and its toxicity on the free cells or immobilized cells was tested. To increase ethanol productivity, extractive fermentation method combined with ethanol fermentation and ethanol recovery was applied to the immobilized batch and continuous fermenter. As the concentration of LaOH was increased, the lag phase became longer, but specific growth rate did not change greatly. And a cell entrapment technique could protect the yeast cells against both substrate inhibition and solvent toxicity. When the glucose concentration was 400 g/l and the LaOH/fermentation medium ratio was 4, total ethanol productivity increased with the enhancement of LaOH volume, and maximum productivity was 2.75 g/l.hr in the immobilized batch fermentation.
Lauryl alcohol을 추출제로 사용한 에탄올 추출발효를 통하여 다음과 같은 결론을 얻을 수 있었다. 1. GC를 이용한 분배계수의 측정은 Fredenslund의 UNIFAC법을 이용한 계산치보다는 고천 낮은 값을 보이나 Minier의 측정치와는 잘 일치하고, 에탄올 농도 2-10%일때 분배계수는 0.6에 달했다. 2. 물과 에탄올은 비점이 비슷하여 95% 이상의 고순도 에탄올을 단증류로 얻기 어렵지만, LaOH는 비점(
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