미생물학회지 (Korean Journal of Microbiology)
- 제27권3호
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- Pages.188-193
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- 1989
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Lactobacillus casei의 phospho-$\beta$ -galactosidase 유전자의 대장균내 분자클로닝
Molecular cloning of phospho-$\beta$ -galactosidase gene of lactobacillus casei in escherichia coli
초록
Gene for lactose catabolism in Lactobacillus casei SW-M1 was encoded by a 60Kb metabolic plasmid. A derivative of only 10kb, pPlac 15 of recombinant plasmid, was constructed by introducing into pBR322 and was cloned into E. coli using restriction endonuclease Pst I. A 10kb insery DNA in plasmid pBR322 was identified as a gene encoded phospho-