Toxicological Research
- Volume 5 Issue 1
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- Pages.9-15
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- 1989
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
EFFECT OF BUTYLATED HYDROXYTOLUENE (BHT) AND ITS METABOLITE ON THE UPTAKE OF TAUROCHOLATE IN PRIMARY CULTURE OF ADULT RAT HEPATOCYTES
- Dong, Mi-Sook (Department of Biologycal Science and Engineering Kaist) ;
-
Choe, Suck-Young
(Department of Food and Nutirition, University of Ulsan)
;
- Yang, Kyu-Hwan (Department of Biologycal Science and Engineering Kaist)
- Published : 1989.06.01
Abstract
The effect of butylated hydroxytoluene (BHT) and its major metabolite, 3, 5-di-tert-butyl-4-hydroxybenzoic acid (BHT-acid) on the uptake of taurocholate into hepatocytes was studied using the primary culture of rat hepatocytes. Hepatocyte were isolated by an in situ collagenase perfusion technique and maintained as a monolayer in serum-free meadia for 24 hours before use. The uptake of taurocholate was saturable with an apparent Km of 12.8+2.8 MuM and Vmax of 0.18+0.01 nmol/mg/min. Both BHT and BHT-acid inhibited the hepatocellular uptake of taurocholate when they were added to the culture.