체외배양한 소난포란과 햄스터척출자궁에서 전배양한 소 정자의 체외수정에 관한 연구

In Vitro Fertilization of Bovine Follicular Oocytes Matured in Culture with Bull Spermatozoa Preincubated in the Uteri Isolated from Hamsters

The present studies were conducted to investigate the possibility of capacitation of bull spermatozoa in the uteri isolated from estrous hamsters and to investigate the possibility of in vitro fertilization of follicular oocytes matured in culture, using ejaculat spermatozoa preincubated in the uteri isolated from estrous hamsters. The follicuar oocytes matured in culture were not fertilized after insemination spermatozoa preincubated for 3 and 5.5-7 h in the uteri isolated from estrou hamsters, but 5, 77, 85 and 50-67% of those oocytes were fertilized by spermatozoa preincubated for 3.5, 4, 4.5 and 5h in those uteri, respectively.