Parasites, Hosts and Diseases
- 제27권3호
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- Pages.187-196
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- 1989
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
감마선 조사가 흰쥐에서의 간흡충 생존 및 발육에 미치는 영향
The effects of gamma irradiation on the survival and development of Clonorchis sinensis metacercariae
초록
우리 나라에서 가장 중요한 인체 기생충의 하나인 간흡충(Clonorchis sinensis)의 감마선에 대한 영향과 그 관리책으로 감마선 조사의 효용성을 관찰하고자 이 연구를 시행하였다. 김해산 참붕어를 수집하여 이를 소화시켜 피낭유충을 분리하고, 분리된 피낭유충을 조사한 실험군(l군), 죽은 참붕어를 조사한 후 피낭유충을 분리한 군(2군), 피낭유충을 흰쥐에 감염시키고 감염 초기에 간을 조사한 군(3군)으로 나누어 실험을 하였다. 조사된 피낭유충은(1,2군) 흰쥐에 실험감염시키고 3군의 실험동물과 함께 감염 2주 및 6주 후에 도살하여 감염된 충체를 회수하였다. 감마선 조사량은 5 Gy(1Gy=100rad)부터 100 Gy의 범위에 있었다. 그 결과를 요약하면 아래와 같다. 1. 분리한 피낭유충을 조사한 후 감염시킨 1군에서는 감염 2주에 평균 충체 회수율이 5, 10, 15 Gy 조사량에 따라 각각 36.5%, 44.1%, 30.7%이었고, 20Gy, 25Gy, 30Gy 조사량에서는 평균 5.7%, 11.8%, 1.0%가 회수되었다. 조사량 50 Gy군에서는 한 마리도 검출되지 않았다. 감염 후 6주의 충체 회수율도 대조군에서 62.6%이 고, 조사량이 증가함에 따라 감소하였고 30 Gy군에서 7.8%, 50 Gy군에서 0%이었다.
The effects of gamma irradiation on the survival and development of C. sinensis metacercariae were studied to evaluate the feasibility of irradiation as a control measure for clonorchiasis. Pseudorasbora parve were collected at an endemic river of clonorchiasis and were used for irradiation of the fluke in three schemes. The first (Scheme 1) was irradiation of the isolated metacercariae from the fish followed by infection to experimental rats. The second (Scheme 2) was irradiation of the fish, and then the metacercariae were isolated and in(ected to rats. The third (Scheme 3) was irradiation on the rat livers after infection with normal metacercariae. Irradiation doses varied from 5 to 100 Gy for Schemes 1 and 2, and 10 to 25 Gy for Scheme 3. The rats were sacrificed 2 to 6 weeks after infection. In Scheme 1, the metacercariae irradiated at 50 Gy failed to survive in the rats after 2 or 6 weeks. However, 1 to 44% of the metacercariae irradiated at 5∼30 Gy survived. The estimated LD50 of Scheme 1 was 16.5 Gy. The flukes irradiated in Scheme 2 survived better than those in Scheme 1. The average worm recovery rate in 50 Gy was 28%(7∼39% individually). Increasing the dose up to 100 Gy brought a remarkably low survival rate of an average 1%(0∼3% individually). The LD50 of Scheme 2 was 47.5 Gy. Worm recovery rates in the 10 Gy group of Scheme 3 were 21∼39%, and those in the 25 Gy group were 2% and 34%. Although the metacercariae were irradiated, all of the recovered worms were morphologically normal. Only the worms irradiated with 10 Gy or 25 Gy after 9 days from infection in Scheme 3 showed underdeveloped testes and seminal receptacle. The present results suggest that irradiation of the fish by 100 Gy could be adopted as a control measure for clonorchiasis.