Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Purification and Chemical Properties of Anti-complementary Polysaccharide from Capsici Fructus

고추의 항보체 다당의 정제와 특성

  • Ra, Kyung-Soo (Department of Food Technology, College of Agriculture, Korea Univ.) ;
  • Yamada, Haruki (Oriental Medicine Research Center of the Kitasato Institute) ;
  • Sung, Ha-Jin (Department of Genetic Engineering, College of Agriculture Univ.) ;
  • Cyong, Jong-Chol (Oriental Medicine Research Center of the Kitasato Institute) ;
  • Yang, Han-Chul (Department of Food Technology, College of Agriculture, Korea Univ.)
  • 나경수 (고려대학교 농과대학 식품공학과) ;
  • 산전양성 (일본 북리연구소 부속 동양의학 종합연구소) ;
  • 성하진 (고려대학교 농과대학 유전공학과) ;
  • 정종철 (일본 북리연구소 부속 동양의학 종합연구소) ;
  • 양한철 (고려대학교 농과대학 식품공학과)
  • Published : 1989.12.30
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Abstract

Water-soluble crude polysaccharide (CAP-0) obtained from the Capsici Fructus(the fruits of Capsium annuum L.) showed a potent anti-complementary activity. The anti-complementary activity did not change by pronase digestion of CAP-0, but decreased by the periodate oxidation. CAP-0 was fractionated into four polysaccharide fractions, CAP-1,2,3 and 4, by the addition of cetyltrimethylammonium bromide. CAP-1, showing the highest anti-complementary activity, was refractioned by anion-exchange chromatography to give three major fractions(CAP-1-III, IV and V). CAP-1-III was shown to have low anti-complementary activity, but CAP-1-IV and V had high activity. CAP-1-III and IV were purified on Sephadex G-100 to give each two fractions(CAP-1-IIIa and IIIb, CAP-1-IVa and IVb), respectively. From the results of gel filtration and electrophoresis, these four fractions and CAP-1-V were found to be homogeneous polysaccharides. High molecular polysaccharies(M.W. CAP-1-IIIa 70,000, IVa 195,000, V 140,000) showed relatively higher anti-complementary activity than low molecular polysaccharides(CAP-1-IIIb and IVb).

고추에서 추출한 수용성 조다당(CAP-0)에 높은 항보체 활성이 존재함을 발견하였으며, 활성은 pronase 처리에 의해 변하지 않았으나 periodate 산화에 의해 활성이 현저히 감소하였다. CAP-0는 cetyltrimethylammonium bromide 처리에 의해 4획분(CAP-1,2,3,4)으로 분리되었으며, CAP-1에서 가장 높은 황보체 활성이 나타났다. CAP-1은 음이온 교환 chromatography에 의해 활성과 수율이 높은 CAP-1-IV와 V로 분리되었으며, III은 수율은 높았으나 활성이 매우 낮았다. CAP-1-III와 IV는 gel 여과에 의해 고분자량 획분$(M.W.\;III_a\;70,000,\;IV_a\;195,000,\;V\;140,000)$과 저분자량 획분 $III_a,\;IV_b$으로 나뉘어졌다. 이 4획분과 V는 gel 여과와 전기영동상에서 순수하였으며 고분자량 획분에서 항보체 활성이 높았다.

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