Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Hematopoietic Characteristics of the Ethylacetate Fraction of Panax ginseng and Identification of the Chemical Constituents

인삼의 에칠아세테이트 추출분획의 조혈활성 특성 및 구성 성분 동정

  • Park, Jong-Dae (Korea Ginseng and Tobacco Research Institute) ;
  • Wee, Jae-Joon (Korea Ginseng and Tobacco Research Institute) ;
  • Kim, Man-Wook (Korea Ginseng and Tobacco Research Institute) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, College of Agriculture, Seoul National University)
  • Published : 1989.06.30
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Abstract

The ethylacetate soluble fraction of Panax ginseng extract, which has been reported to exhibit hematopoietic effect, was divided into three subfractions, i.e., Fr. I, Fr. II and Fr. III by silica gel column chromatography. The hematopoietic effect of each subfraction was examined in rats treated with anticancer agent, Ara-C($1-{\beta}-D-arabinofuranosylcytosine)$). Among them, Fr. III showed 90% recovery of leucocyte and erythrocyte counts in bonemarrow cell depleted rats, suggesting it to be hematopoietic fraction. From Fr. III, two major compounds were isolated and identified as ginsenoside $Rh_1$ and $Rg_2$ by $^{13}C-NMR$ and degradation method.

인삼 에칠아세테이트 추출 분획을 silica gel column chromatography하여 Fr. I, Fr. II 및 Fr. III으로 나눈 다음 항암제 투여로 조혈기능이 억제된 흰쥐에 투여해 본 결과 Fr. III이 감소된 백혈구 및 적혈구 수를 90%까지 회복시켰다. Fr. III에서 두성분을 분리하여 구조를 동정한 결과 ginsenoside $Rh_1$ 및 $Rg_2$로 확인하였다.

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