Journal of Pharmaceutical Investigation
- Volume 19 Issue 2
- /
- Pages.81-86
- /
- 1989
- /
- 2093-5552(pISSN)
- /
- 2093-6214(eISSN)
Studies on Higher Fungi in Korea (III)-Purification and Stability of Proteolytic Enzyme in Sarcodon aspratus (Berk.) S. Ito-
한국산 고등균류에 관한 연구(제 3보)-능이 중의 단백질 가수분해효소의 정제 및 안정성-
- Lee, Tae-Kyoo (Jeonju Woosuk University) ;
-
Eun, Jae-Soon
(Jeonju Woosuk University)
;
-
Yang, Jae-Heon
(Jeonju Woosuk University)
;
- Jo, Duck-Yi (Jeonju Woosuk University) ;
- Yang, Hee-Cheon (Jeonju Woosuk University)
- Published : 1989.06.20
Abstract
The proteolytic enzyme extracted from Neungee [Sarcodon aspratus (Berk.) S. Ito] was purified by using Tris-acryl CM-cellulose column chromatography and chromatofocusing. The specific activity of the purified enzyme increased 15.8 times as compared with that of the crude enzyme. The enzyme was homogeneous on polyacrylamide gel electrophoresis and stable at pH values ranging from 4.0 to 10.8. The enzyme activity remained unchanged when the mushroom and the purified enzyme were stored for 3 years and 6 months at 4°C, respectively. The enzyme was found to be an endogeneous protease.