한국균학회지 (The Korean Journal of Mycology)
- 제17권3호
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- Pages.161-168
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- 1989
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
무흡광색소 생물의 감광수용체 개발연구(V) - 표고버섯 중의 광감응성 Mitochondrial ATPase 및 ATP synthase에 대한 FAD 및 $FADH_2$ 의 효과 -
Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (V) - Effects of FAD and $FADH_2$ on Light-Induced Mitochondrial ATPase and ATP Synthase in Lentinus edodes -
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Park, Sang-Shin
(Department of Chemistry, College of Sciences, Dongguk University) ;
- Min, Tae-Jin (Department of Chemistry, College of Sciences, Dongguk University)
- 발행 : 1989.09.30
초록
표고버섯(L. edodes) 중의 mitochondria는 설탕밀도단계기울기법에 따라 분리정제 하였다. 앞서 보고한 바와 같이, 각 파장별 빛조사(400-700nm)에 따른 mitochondrial ATPase와 ATP synthase의 활성도는 680nm와 470nm에서 각각 활성화되었다. 본 연구에서, 400nm 이하의 파장별 빛조사에 따른 mitochondrial ATPase 및 ATP synthase의 활성도는 380nm와 330nm에서 각각 활성화되었으며, 330nm 및 350에서 각각 억제되었다. FAD의 존재하에서, mitochondrial ATP synthase는 활성화 파장 및 억제 파장의 조사에 의하여 활성도가 각각 증가된 반면, mitochondrial ATPase의 활성도는 감소되었다. 그러나, NADH의 존재하에서, 이들 파장의 조사에 의한 효소의 활성도는 변화가 없었다. 또한, 두 효소는 각각의 활성화 파장 및 억제 파장이 조사됨에 따라
Mitochondria in L. edodes were separated and purified by stepped sucrose density gradient centrifugation. In our previous work, we have found that the activation wavelengths of the mitochondrial ATPase and ATP synthase were 680 nm and 470 nm within the range of 400-700 nm, respectively. The activities of the above enzymes with wavelengths of 300-400 nm region were investigated. The mitochondrial ATPase and ATP synthase were stimulated at 380 nm and 330 nm, respectively, for 30 min illumination compared with dark control group. They, however, were inhibited at 330 nm and 350 nm, respectively. The presence of FAD resulted in inhibition of the activity of the ATPase and stimulation of the activity of the ATP synthase by the activation and inhibition wavelengths. However, the activities of these enzymes were not changed by NADH for the above wavelengths. In the spectral properties, the oxidation of