대한약리학회지 (The Korean Journal of Pharmacology)
- 제25권1호
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- Pages.31-42
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- 1989
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- 0377-9459(pISSN)
흰쥐 적출 부신에서 Metoclopramide의 Catecholamine 분비작용에 관한 연구
Studies on Secretion of Catecholamines Evoked by Metoclopramide of the Rat Adrenal Gland
- 임동윤 (조선대학교 의과대학 약리학교실) ;
- 김규형 (조선대학교 의과대학 약리학교실) ;
- 최철희 (조선대학교 의과대학 약리학교실) ;
- 유호진 (조선대학교 의과대학 약리학교실) ;
- 최동준 (조선대학교 의과대학 약리학교실) ;
- 이은화 (조선대학교 의과대학 약리학교실)
- Lim, Dong-Yoon (Department of Pharmacology, College of Medicine, Chosun University) ;
- Kim, Kyu-Hyeong (Department of Pharmacology, College of Medicine, Chosun University) ;
- Choi, Cheol-Hee (Department of Pharmacology, College of Medicine, Chosun University) ;
- Yoo, Ho-Jin (Department of Pharmacology, College of Medicine, Chosun University) ;
- Choi, Dong-Joon (Department of Pharmacology, College of Medicine, Chosun University) ;
- Lee, Eun-Hwa (Department of Pharmacology, College of Medicine, Chosun University)
- 발행 : 1989.06.30
초록
Metoclopramide (MCP)는 역류성식도염, 위하수증, 항암제에 의한 구토 증상을 치료하는데 사용되고 있는 dopamine수용체 차단제로 알려져 있다. 본 연구에서는 흰쥐의 적출 관류부신에서 catecholamine (CA)의 분비작용에 미치는 영향을 검토하여 다음과 같은 결과를 얻었다. MCP는 부신정맥내로 주입하였을때 용량의존적으로 CA 분비작용을 나타냈다. 이러한 MCP의 CA 분비작용은 atropine 전처치로 차단되었으나 chlorisondamine 처치에 의해서는 완전 차단되지 못하였다. MCP 분비작용은 physostigmine, adenosine, ouabain의 전처치로 현저히 증강되었다. 그러나 5mM-EGTA 함유
The effect of metoclopramide (MCP), which is well-known as a selective dopaminergic antagonist used in treating esophageal refulx, gastroparesis and emesis induced by anticancer chemotherapy, on secretion of catecholamines (CA) in the perfused isolated rat adrenal gland was investigated. MCP given into an adrenal vein produced the dose-related increase in CA secretion from the adrenal gland. The secretory effect of CA evoked by MCP was inhibited markedly by atropine-pretreatment. but only partially blocked when chlorisondamine was added. The secretion of CA induced by MCP was potentiated by pretreatment with physostigmine, adenosine or ouabain. However, MCP-induced CA secretion was suppressed significantly by perfusion of calcium-free Krebs solution containing 5 mM-EGTA for 30 min. Perfusion of MCP (200 ug/30 min.) attenuated the secretory effect of CA evoked by potassium chloride or acetylcholine. These experimental results demonstrate that metoclopramide releases CA significantly by a calcium-dependent exocy totic mechanism. It is thought that the secretory effect of metoclopramide is due to activation of cholinergic muscarinic receptors present in the adrenal gland rather than nicotinic receptors and partly to the direct action on the chromaffin cell itself.