YAKHAK HOEJI (약학회지)
- Volume 33 Issue 6
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- Pages.365-371
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- 1989
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Production of 1,4-Androstadiene-3,17-dione by a Mutant Strain of Brevibacterium lipolyticum
Brevibacterium lipolyticum 변이주에 의한 1,4-Androstadiene-3, 17-Dione의 생성
- Choi, In-Wha (College of Pharmacy, Ewha Womans University) ;
- Lee, Kang-Man (College of Pharmacy, Ewha Womans University)
- Published : 1989.12.28
Abstract
Microbiological conversion of sterols to 17-ketosteroids has been recognized as a source for commercial preparation of steroidal drugs. In order to develop bacterial strains and process with Brevibacterium lipolyticum IAM 1398 capable of converting cholesterol to 1,4-Androstadiene-3,17-dione (ADD) at about 27% yield, we studied on strain improvement, fermentation condition and whole cell immobilization. By using UV and/or NTG as mutagens, a mutant to convert cholesterol to ADD with higher yield than 60% was selected. Better production of ADD was manifested in the case of maltose used as a supplemental carbon source, and yeast extract or soytone as a nitrogen source. Addition of tween 80 (0.05%) as a surfactant beneficial for increasing the productivity. The optimal initial pH of the medium was 6.5 and optimal culture temperature was