YAKHAK HOEJI (약학회지)
- Volume 33 Issue 3
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- Pages.156-160
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- 1989
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Determination of Thiamin by Gas-chromatography
기체크로마토그래피법에 의한 티아민 분석
- Yoo, Ji-Sang (College of Pharmacy, Chungbuk National University) ;
- Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University) ;
- Hong, Sung-Hwa (College of Pharmacy, Chungbuk National University) ;
- Han, Kun (College of Pharmacy, Chungbuk National University) ;
- Kim, Bak-Kwang (College of Pharmacy Seoul National University)
- Published : 1989.06.28
Abstract
A gas-chromatographic determination method of thiamin which use a quantitative cleavage of thiamin to 4-methyl-5-(2-hydroxyethyl)thiazol [I] and solvent extraction of the analyte prior to GC injection was modified. A column chromatographic procedure using a reversed phase, high capacity solid phase cartridge was applied to the clean-up of the analyte. Thiazol derivative[I] was quantitatively recovered upon the column method. Acetanilide, an internal standard, has a good recovery through the analytical procedure. The method has analytical precision of 2% or less in the coefficient of variation.