Abstract
The study was intended to know any relations between the rice tolerance to bensulfuron and varietal speciation in seed protein composition or any enzymatical allelies with or without chemical treatment. Rice varieties used were UCP-28, Chinsurah Boro II, Fukunohama, Fadehpur-2, IR 14252-13-2-2-5 as the tolerant group, and HP 93(3) FA, HP94(9) FA, Padilabou Alumbis, KH-17854, and IR 1846-2841-1 as the susceptible, respectively. Electrophoretic methods used were SDS-PAGE for seed protein, 7% PAGE for isozymes (acid phosphatase, peroxidase, malate dehydrogenase, and esterase from rice seedling) and variation in isoenzyme profiles (malate dehydrogenase, peroxidase, and esterase) as affected by different concentrations of bensulfuron(0, $10^{-6}$, $10^{-5}$ and $3{\times}10^{-5}M$) was also studied. The results are summarized as follows. -Among 16 bands separated in seed proteins, two different rice groups selected in terms of tolerance to bensulfuron were clustered in dissimilarity, which was based on relatively larger area in whole peaks and higher activities in N, O, P bands for the tolerant group. -Among isozymes obtained from rice seedlings without chemical treatments, the following specificities were obtained. The tolerant varieties had the relatively higher activity in D band out of 4 peroxidase bands. Malate dehydrogenase was separated into 3 bands and only tolerant varieties had A band and higher activities in Band C bands. Esterase was separated into 3-4 bands with higher activities in A and B bands for tolerant varieties. There were one major band accompanied by 2-3 minor bands for acid phosphatase in which only tolerant varieties had the B band. -The effect of Bensulfuron concentration on the isozyme activities showed that the activity of C band in peroxidase was not present in tolerant varieties which was contrary to the increased activities in susceptible varieties. However, D band was gradually disappeared only in susceptible varieties as the concentration of bensulfuron was increased. For malate dehydrogenase in the susceptible varieties, major bands D, E and F kept consistantly higher activities while minor bands A, B and C disappeared sensitively. Among 5 bands of esterase separated, D band was present only in the tolerant varieties while E band only in the susceptible. The activities in A, C, E bands were sharply decreased in the susceptible varieties as the concentration of bensulfuron was increased.
Bensulfuron에 대표적인 내성군(耐性群)(UCP-28, Chinsurah Boro II, Fukunohama, Fadehpur-2, IR 14252-13-2-2-5)과 감수성품종군(感受性品種群)(HP93(3) FA, HP94(9) FA, Padilabou Alumbis. KH-17854, IR 1846-2841-1)을 공시(供試)하여 종실(種實) 단백질(蛋白質)(SDS-PAGE)과 유묘(幼苗) 동위효소(同位酵素)(malate dehydrogenase, acid phosphatase, peroxidase, esterase: 7% PAGE)의 전기영동(電氣泳動) profile을 분석(分析)하고, bensulfuron 0, $10^{-6}$, $10^{-5}$, $3{\times}10^{-5}M$ 용액에서 생장(生長)한 두 대표 품종(品種)(cv. Chinsurah Boro II와 cv. IR 1846)의 5 일령(日齡) 유묘(幼苗)의 동위효소(同位酵素) profile 변화양상(變化樣相)을 비교(比較)하므로써, bensulfuron에의 내성차이(耐性差異)와 이들 전기영동(電氣泳動) profile 차이(差異)가 갖는 연관성(聯關性)을 알고저 하였다. 결과(結果)를 요약(要約)하면 다음과 같다. 1. 종실단백(種實蛋白)의 전기영동(電氣泳動)으로 16개(個) band를 확인(確認)할 수 있었고, 이를 근거(根據)로 clustering한 결과(結果), 내성(耐性)에서의 M, O, P band가 높게 나타났고 또한 전체 peak 면적(面積)이 크다는 특이성(特異性)에 기인(起因)하여 두 품종군간(品種群間)에 비유사성(非類似性)이 인정(認定)되는 정도(程度)로 분류(分類)되었다. 2. 무처리(無處理)된 유묘(幼苗)의 동위효소(同位酵素) 가운데, peroxidase로는 4개(個) band가 얻어졌고, 내성군(耐性群)의 특이성(特異性)은 D band에서의 활성(活性)이 높은 데 있었다. Malate dehydrogenase는 3개(個) band로 분리(分離)되었고, 내성군(耐性群)만이 A band를 나타내며 B 및 C band도 내성군(耐性群)에서 선명하였다. Esterase는 3~4개(個) band로 구분(區分)되었고 내성군(耐性群)이 A 및 B band에서 높은 활성(活性)을 보였다. Acid phosphatase는 하나의 major band와 2~3개(個)의 minor band로 나타났으며 내성군(耐性群)만은 B band를 갖는 경향(傾向)이었다. 3. Bensulfuron의 처리농도(處理濃度)에 따라, 동위효소(同位酵素) 가운데 peroxidase는 감수성(感受性)에서만 C band의 생성(生成)과 D band의 소실현상(消失現象)이 야기(惹起)되었고, 내성품종(耐性品種)에서는 C band가 나타나지 않았으며, 다른 band에서의 변화(變化)도 없었다. Malate dehyrogenase의 경우, major band인 D, E, F는 감수성(感受性)에서 활성(活性)이 컸고 변화(變化)는 없었으며, minor band인 A, B, C는 감수성(感受性)에서만 민감(敏感)하게 소실(消失)되었다. Esterase는 5개(個) band로 표현(表現)되었으며 내성(耐性)에서는 A, B, C, D 감수성(感受性)에서는 A, B, C, E만 나타났으며, 약처리농도증가(藥處理濃度增加)로 감수성품종(感受性品種)은 A, C, E band의 활성(活性)이 민감(敏感)하게 소실(消失)되었다.
