Immunohistochemistry for detection of Aujeszky's disease virus antigens : Immunogold-silver method in tissue sections

오제스키병 바이러스 항원검출을 위한 면역조직화학적 연구 -조직절편내 immunogold-silver법-

The present study was done to demonstrate ADV antigens in frozen and paraffin sections from ADV-infected pigs and cell cultures by using of the IGS method. Tissue specimens from 3 young pigs infected with ADV-phylaxia strain and of 2 healthy pigs were used. Fibroblastic cells originated from pig brain and BHK cells were grown and confluent monolayers were infected with the virus. Two monoclonal antibodies and a specific hyperimmune serum to ADV were used as the source of primary antibodies for both the IGS and immunoperoxidase methods. Application of the IGS method yielded a black fine granular reaction in positive areas, and the results were superior to those obtained using the immunoperoxidase technique for all cases tested. The IGS method might be useful in the detection of various viral antigens in tissue sections.

본 연구는 감염돼지와 조직배양세포의 동결 및 파라핀 절편에서 immunogold-silver(IGS)법을 이용하여 오제스키병 바이러스를 검출코자 하였으며, 이를 위해 자돈 5두와 돼지뇌유래 섬유 아세포 및 BHK 세포를 공시하였고, IGS법과 immunoperoxidase법의 1차 항원으로는 2종의 단크론성 항체와 고도면역혈청을 사용하였다. IGS법은 양성부위에서 흑색의 미세한 과립의 침착을 볼 수 있었고, immunoperoxidase법에 비해 탁월한 효과를 얻었으며, IGS법은 조직내에 존재하는 각종 바이러스 항원을 검출하는데 이용할 수 있을 것으로 생각된다.