한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)
- 제5권1호
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- Pages.25-30
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- 1988
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- 1225-9098(pISSN)
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- 2288-1069(eISSN)
DOI QR Code
Ferrothiocyanate법에 의한 혈청 Lipid Hydroperoxide정량
Quantitative Determination of Lipid Hydroperoxide in Human Blood Serum by Ferrothiocyanate Method
- Paik, Taik-Hong (Dept. of Chemistry, Hanyang University) ;
- Park, Chan-Sik (Dept. of Chemistry, Hanyang University) ;
- Chun, Hyun-Ja (Dept. of Chemistry, Hanyang University)
- 발행 : 1988.05.31
초록
In order to develope the method of quantitative determination of lipid hydroperoxide in human blood serum, we tried the ferrothiocyanate method to total lipids extracted by Bligh-Dyer method and obtained the results as follows. 1. The maximum absorbance showed at the concentration of Mohr's solution, 0.127M at pH 1.70 and ammonium thiocyanate solution, 3.95M in the ferrothiocyanate method. 2. When hydrogen peroxide, cumene hydroperoxide, and oxidized linoleic acid were added to serum, and extracted them by Bligh-Dyer method to examine the extraction efficiency, we confirmed that cumene hydroperoxide and oxidized linoleic acid were extracted in
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