Applied Microscopy
- Volume 18 Issue 2
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- Pages.167-176
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- 1988
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Electron Microscopic Study on Extrachromosomal DNA from Splenocytes and Erythrocytes of Carassius carassius L.
붕어의 Splenocyte 및 Erythrocyte의 Extrachromosomal DNA 관찰
- Im, Sook-Ja (Dept. of Biology, Korea University) ;
- Kim, Woo-Kap (Dept. of Biology, Korea University)
- Published : 1988.12.01
Abstract
Extrachromosomal circular DNA complexes from erythrocytes and splenocytes isolated from Carassius carassius were examined by mica-press-absorption method. The method was described that released small polydisperse circular DNA molecules in situ from the erythrocytes and the splenocytes and that allows selective observation of the small circular DNA complexes bound to cellular components. The released polydisperse circular DNA complexes were absorbed preferentially on mica in a divalent cation-free medium then processed for electron microscopy. Small circular DNAs showed a heterogeneous size distribution of
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