Korean Journal of Poultry Science (한국가금학회지)
- Volume 15 Issue 3
- /
- Pages.199-206
- /
- 1988
- /
- 1225-6625(pISSN)
- /
- 2287-5387(eISSN)
Production of Monoclonal Antibody to Infectious Laryngo- tracheitis Virus by Cell Fusion
닭 전염성 후두기관염 바이러스에 대한 단크론성 항체생산
- Chung Ok Choi (College of Veterinary Medicine, Chonnam National University) ;
- Chung Gil Lee (College of Veterinary Medicine, Chonnam National University) ;
- Sung Man Cho (College of Veterinary Medicine, Chonnam National University) ;
- Soo Hwan An (Institute of Veterinary Research) ;
- Joon Hun Kwon (Institute of Veterinary Research)
- Published : 1988.09.01
Abstract
A total of 3 hybridoma clones producing monoclonal antibody (MCA) against infectious laryngotracheitis virus (ILTV) was established by somatic cell hybridization between mouse myeloma cells and spleen, cells from mice immunized with ILTV. The MCAs were screened by the indirect flourescent antibody (IFA) staining and the specific hybridomas were cloned by limiting dilution method. The MCAs produced by the 3 hybriomas were all classified as immunogloblin G and found to be reacting against common antigen(s) of high and low pathogenic ILTV examined. The titer of these antibodies in mouse ascitic fluid was from
국내에서 분리한 강독전염성후두기관염 바이러스 (ILTV)에 대한 세포융합방법에 의해 단크론성항채(MCA) 생산을 시도한 결과 총 8회의 세포융합을 통하여 총 1017개의 융합세포가 생산되었으며 그중 ILTV와 특이적으로 작용하늘 항체를 생산하는 3주의 Hybridoma를 작성하였다. 이 3주의 MCA는 모두 IgG형에 속하였으며 마우스 복강내접종하여 생산된 복수항체외 형광항체가는
Keywords