한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제16권3호
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- Pages.193-198
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- 1988
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Aspergillus niger KUF-04가 생산하는 Catalase
Catalase from Aspergillus niger KUF-04
- Yang, Ho-Suk (Department of Food Technology, Korea University) ;
- Yang, Han-Chul (Department of Food Technology, Korea University) ;
- Yoshiki Tani (Department of Agricultural Chemistry, Kyoto University)
- 발행 : 1988.06.01
초록
Aspergillus niger KUF-04에서 얻은 catalase는 gel 여과를 포함하여 5단계를 걸쳐 정제하였으며, 9% 회수율로 64배 정제되었다. 본 효소는 406, 503, 625nm에서 흡광을 나타내었으며, 특히 406nm에서 뚜렷한 흡수대를 보여주었다. 이 효소 활성의 최적 pH와 온도는 각각 7.0과 6
Catalase from Aspergillus niger KUF-04 was purified by five steps including gel filtration. The overall purification gave 64-fold purified preparation, a yield of about nine percent. The enzyme showed its maximum absorption at 406 nm. The optimum pH and temperature for the enzyme activity were around pH 7.0 and 6