한국식품영양학회지 (The Korean Journal of Food And Nutrition)
- 제1권2호
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- Pages.50-55
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- 1988
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
고구마 $\beta$ -아밀라제의 subunit
Subunits of Sweet Potato $\beta$ -Amylase
초록
B-Amylase was obtained from sweet potato extract in a crystalline state by dialysis against water after precipitated with acetone according to the method reported previously followed by DEAE Sephadex A-50 ion exchange chromatography plus gel chromatography of Sephadex G-200. The purified enzyme was homogeneous by SDS PAGE. The efforts had done to remove the miner bands in SDS PAGE by Sephadex G-200 gel chromatography, DATE Sephadex A-50 ion exchange chromatography. isoelectrophocusing, affinity chromatography, hydroxyapatite chromatography, recrystallizstion and HPLC on a column of TSK gel SW 3000 but have given any result. But, N -terminal amino acid of the enzyme was revealed mainly alanine and trace of glycine and glutamic acid. Therefore, it seems that the miner bands in SDS PAGE have a role of subunit.
키워드