미생물학회지 (Korean Journal of Microbiology)
- 제26권2호
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- Pages.82-87
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- 1988
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Candida pseudotropicalis 융합세포의 유전적 분석
Genetic analysis of protoplast fusants of candida pseudotropicalis
- Chun, Soon-Bai (Department of Biology, College of Natural Sciences, Chonnam National University) ;
- Bai, Suk (Department of Biology, College of Natural Sciences, Chonnam National University)
- 발행 : 1988.06.01
초록
The genetic analysis and characterization of protoplast fusion hybrids between complementary auxotrophic mutants of Candida pseudotropicalis were carried out. Nuclear fusion appeared to occur in fusion hybrids (e.g., F15 and F33), as strongly suggested by isolation of recombinants after mitotic segregation of parental genetic markers. This was confirmed by KNA content, nuclear staining and comparison of survival rate to UV light. After keeping fusion hybrids for approximately one year, the frequency of spontaneous mitotic segregation was