Studies on Simplified Procedures for Freezing and Thawing of Bovine Embryos VII. Effects of freezing procedures in a liquid nitrogen container on the survival rate of mouse embryos

육우수정란 간역동결 및 융해방법에 관한 연구 제칠보. 내동제에 Sucrose 첨가에 따른 액체질소에 미치는 영향

This study was done with mouse embryos to determine effects of the freezing media with or without 10% sucrose, and seeding methods (pincette, no seeding, liquid nitrogen gas phase and copper wire coiled straw) on embryo survival were determined using the FDA test. The summarized results are the following. 1. The FDA score found with copper wire coiled straw, no seeding, pincette and liquid nitrogen gas phase was 3.6, 3.6, 3.3 and 3.0, respectively. There were no significant differences. 2. The embryo score shows higher (P<0.05) survival rate using a freezing medium with sucrose than the one without it. Among the seeding procedure, better resutls are copper wire coiled straw and no seeded. 3. The results suggest that copper wire coiled seeding no seeding be as good as seeding when the mouse embryos were frozen in a liquid nitrogen container using both the freezing and dilution media containing 10% sucrose.