육우수정란 간역동결 및 융해방법에 관한 연구 제5보. Glycerol 내동제에 Sucrose 첨가여부가 FDA Test에 의한 Mouse 수정란의 생존율에 미치는 영향

Studies on Simplified Procedures for Freezing and Thawing of Bovine Embryos V. Effects of the glycerol cryoprotectants containing sucrose on the mouse embryo survival rate determined by FDA test

Effects of the glycerol addition and removal medium containg 10% sucrose on the mouse embryo survival after freezing in a liquid introgen container were determined using the FDA test. The summarized results are the following. 1. The FDA score was higher (P<0.05) when embryos were frozen in the glycerol addition medium with sucrose that the one without it(3.4 vs 3.0). 2. No difference in the score was found between the glycerol removal medium containing 10% sucrose(3.2) and PBS＋10% sucrose(3.3). 3. The score was higher(P<0.01) at morular stage than blastocyst stage of embryos.