Applied Biological Chemistry
- 제31권4호
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- Pages.331-338
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- 1988
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
칡 뿌리의 Polyphenol Oxidase의 정제 및 성질에 관한 연구
Purification and Some Properties of Polyphenol Oxidase from Arrowroot
- Oh, Man-Jin (Department of Food Science and Technology, College of Agriculture, Chungnam National University) ;
- Lee, Won-Yong (Department of Food Science and Technology, College of Agriculture, Chungnam National University) ;
- Lee, Ka-Soon (Department of Food Science and Technology, College of Agriculture, Chungnam National University)
- 발행 : 1988.12.30
초록
칡뿌리로 부터 칡뿌리 acetone분말을 제조하고 polyphenol oxidase를 추출, 정제하여 정제효소의 효소학적 특성을 검토하였다. 조효소는 DEAE-Cellulose, DEAE-Sephadex A-50, Sephadex G-100 column chromatography에 의하여 정제되었으며, 정제효소의 비활성은 94배, 정제수율은 45.4%이었다. 정제효소는 catechol 및 pyrogallol에 대하여 감한 친화성을 나타내었으며, km 값은 catechel을 기질로 하였을 때 16.67mM이었다. 작용 최적 pH는 7.5, 최적온도는
Acetone powder was prepared from raw arrowroots and the polyphenol oxidases of crude enzyme prepared from acetone powder were identified 5 isoenzymes by staining with catechol containing 0.05% phenylene diamine. The crude enzyme was passed through the columns of ion exchangers and gel permeation to fractionate the polyphenol oxidases. The main fraction of polyphenol oxidase appeared to be purified by 94-fold, with the activity yield of 45.4%, and its molecular weight was determined as 38,500 by poly acrylamide gel electrophoresis. The optimal pH and temperature for the enzyme activity were pH 7.5 and
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