Applied Biological Chemistry
- 제31권3호
- /
- Pages.219-225
- /
- 1988
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
역전사효소(逆轉寫酵素) 유전자(遺傳子)의 cloning 에 관(關)한 연구(硏究)
Cloning of Reverse Transcriptase Gene of Avian Sarcoma Virus
- Kim, Yong-Woong (Department of Agricalural Chemistry, Chonnam National University) ;
- Kim, Kwang-Sik (Department of Agricalural Chemistry, Chonnam National University) ;
- Suh, Yong-Tack (Department of Agricalural Chemistry, Chonnam National University) ;
- Guntaka, R.V. (Department of Microbiology, University of Missouri Columbia)
- 발행 : 1988.09.30
초록
Avian Sarcoma Virus의 plasmid DNA중(中)의 역 이사효소의 유전자(遺傳子)를 온도의존성(溫度依存性) 발현(發現) vector인 pPL-lambda에 cloning하여 온도(溫度)에 민감한 phage
Reverse transcriptase gene of Avian sarcoma virus(ASV) was cloned with a thermoinducible expression vector, pPL-lambda. E. coli N4830 which carries temperature sensitive cI857 la mbda repressor, was transformed with this pPL-pol plasmid DNA. The RNA transcribed by those tranoformants was isolated and analyzed. It was shown that the inserted reverse transcriptase gene of ASV was transcribed at high-level when cells were grown at high temperature.
키워드