Journal of Pharmaceutical Investigation
- Volume 18 Issue 3
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- Pages.125-131
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- 1988
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Studies on Higher Fungi in Korea (I) -Activity of Proteolytic Enzyme from Sarcodon aspratus (Berk) S. Ito-
한국산 고등균류에 관한 연구(제 1보) -능이버섯의 단백분해효소 활성-
- Eun, Jae-Soon (Jeonju Woosuk University) ;
- Yang, Jae-Heon (Jeonju Woosuk University) ;
- Cho, Duck-Yee (Jeonju Woosuk University) ;
- Lee, Tae-Kyu (Jeonju Woosuk University)
- Published : 1988.09.20
Abstract
This study was undertaken to investigate the proteolytic enzyme from Neungee mushroom [Sarcodon aspratus (Berk) S. Ito]. The proteolytic activity of Neungee was higher than other several edible mushrooms under various pHs. The potency of proteolytic enzyme of Neungee was same as the digestive drugs containing protease. So the proteolytic activity of the enzyme was increased in neutral or weak alkaline pH, whose characteristics would be alkaline protease. The specific activity of the purified enzyme obtained by using Tris acryl CM-cellulose ion exchange increased 20 times as compared with that of the crude extract. The proteolytic enzyme was stable at room temperature, but decomposition was fast when incubated at higher temperature more than
Keywords
- Neungee mushroom;
- Sarcodon aspratus (Berk) S. Ito;
- alkaline protease;
- proteolytic enzyme activity;
- purification;
- stability