한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제20권4호
- /
- Pages.547-552
- /
- 1988
- /
- 0367-6293(pISSN)
Chitosan 고정화 Aminoacylase 를 이용한 DL 아미노산의 광학적 분할에 관한 연구 : 고정화 Aminoacylase의 성질 및 반응성
Studies on the Optical Resolution of DL-Amino Acids by Aminoacylase Immobilized on Chitosan: Properties and Reactivity of Immobilized Aminoacylase
- Lee, Sang-Hyun (Department of Food Science and Technology Chung-Ang University) ;
- Lee, Young-Chun (Department of Food Science and Technology Chung-Ang University)
- 발행 : 1988.08.01
초록
고정화 aminoacylase의 N-ac D Met N-ac DL Try 및 N-ac DL Phe에 대한최적 pH는 각각 8.0, 7.0, 7.5이었고. pH 안정성은 가용성 효소에 비해 감소하였다. 고정화 효소의 열 안정성은 가용성 효소와 별 차이가 없었고, 최적온도는
Aminoacylase immobilized on chitosan was applied for optical resolution of DL-amino acids. Optimun pH's for hydrolysis of N-ac DL Met, N-ac DL Try and N-ac DL Phe by immobilized aminoacylase were 8.0, 7.0, and 7.5, respectively. The pH stability of immobilized aminoacylase was less than that of soluble enzyme, while there was no difference in thermostability between immibilized and soluble enzymes. The reaction rate of immobilized enzyme was maximum, when concentrations of N-ac DL Met, N-ac DL Try and N-ac DL Phe were 0.05, 0.03 and 0.05M, respectively. Continuous resolution of M/20 N-ac DL amino acids with immobilized aminoacylase packed in a column resulted in 100% hydrolysis upto space velocity