배양조건에 따른 도열병균(Pyricularia oryzae) 균사체의 Esterase 동위효소

Esterase Isozyme of Mycelium of Pyricularia oryzae under Various Cultural Conditions

도열병균(Pyricularia oryzae) 균사체의 esterase 동위효소 pattern이 배양조건에 의하여 변화되는가를 $10\~25\%$ polyacrylamide gradient slab gel을 사용하여 알아보았다. 배양기간에 따라 배양 24일까지 동위효소 pattern은 변하지 않았지만 특징 band의 효소량에는 차이가 있었다. 탄소원(glucose, fructose, maltose, sucrose, starch), 단포자분리균주간, 계대배양 및 세포벽재생균주간의 동위효소 pattern은 변함이 없었다.

The constancy of the patterns of esterase isozymes in the mycelium of Pyricularia oryzae grown under various cultural conditions was observed by $10\~25\%$ polycrylamide gradient slab gel. The position of the isozyme was not affected by cultural age for 24 days. However the intensity of the band was affected. The band patterns were not affected by the carbon sources (glucose, maltose, fructose, sucrose or starch), single spore isolates, successive transfer culture and regenerated isolates from protoplast cultures.