Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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The DNA Damage by Linoleic Acid Hydroperoxide

Linoleic acid과산화물의 DNA 손상작용

  • KIM Seon-Bong (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • KANG Jin-Hoon (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • BYUN Han-Seok (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • KIM In-Soo (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • PARK Yeung-Ho (Department of Food Science and Technology, National Fisheries University of Pusan)
  • 김선봉 (부산수산대학 식품공학과) ;
  • 강진훈 (부산수산대학 식품공학과) ;
  • 변한석 (부산수산대학 식품공학과) ;
  • 김인수 (부산수산대학 식품공학과) ;
  • 박영호 (부산수산대학 식품공학과)
  • Published : 1987.11.01
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Abstract

The DNA damage by linoleic acid hydroperoxide (LHPO) was investigated in a DNA-LHPO system at $37^{\circ}C$ to elucidate the DNA damage mechanism by lipid peroxidation products. LHPO shelved a great DNA damage with the increase of its concentrations. DNA was completely damaged in a LHPO-DNA(weight ratio, 2:3) system after incubation for 2 days. The degree of DNA ,damage by LHPO was greated than that of linoleic acid. In the quantitative analysis of DNA damage, the decreasing ratio of DNA content was $60\%$ in $84{\mu}g$ LHPO system incubated for 1 day compared to the control solution marked $30\%$. There were no participation of active oxygens on the DNA damage by LHPO.

지질산화생성물의 DNA 손상작용기구를 밝힐 목적으로 자동산화시킨 linoleic acid로부터 과산화물을 분리하고 DNA와 $37^{\circ}C$에서 반응시켜 경시적인 관산화물이 DNA 원상작용을 조사한 결과는 다음과 같다. 과산화물의 농도가 증가함에 따라 DNA가크게 송상되었으며, 반응 2일째에는 $422{\mu}g$ 이상의 농도에서는 DNA가 더욱 크게 손상을 받아 gel plate 상에서는 DNA band가 검출되지 않았다. 또, 이러한 DNA 손상능을 정량적으로 분석한 결과, 반응 1일에 대조구가 $30\%$의 감소율을 나타낸 반면, $844{\mu}g$의 농도에서는 2배량인 $60\%$의 감소율을 나타내었다. 과산화물과 DNA의 반응계에 각종 활성산소소거제를 첨가한 결과, 과산화물의 DNA 손상작용에 대한 활성산소종의 영향은 나타나지 않았다.

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