Applied Microscopy
- Volume 17 Issue 1
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- Pages.47-64
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- 1987
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
An Ultrastructural Study on the Renal Glomeruli of the Aging Rat
연령에 따른 흰쥐 신사구체의 변화에 대한 전자현미경적 연구
- Yang, Nam-Gil (Dept. of Anatomy, College of Medicine, Soonchunhyang University) ;
- Han, Jong-Won (Dept. of Anatomy, College of Medicine, Soonchunhyang University) ;
- Ahn, Eui-Tae (Dept. of Anatomy, College of Medicine, Soonchunhyang University) ;
- Ko, Jeong-Sik (Dept. of Anatomy, College of Medicine, Soonchunhyang University) ;
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Park, Kyung-Ho
(Dept. of Anatomy, College of Medicine, Soonchunhyang University)
- 양남길 (순천향대학교 의학부 해부학교실) ;
- 한종원 (순천향대학교 의학부 해부학교실) ;
- 안의태 (순천향대학교 의학부 해부학교실) ;
- 고정식 (순천향대학교 의학부 해부학교실) ;
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박경호
(순천향대학교 의학부 해부학교실)
- Published : 1987.05.01
Abstract
Morphological difference of the renal glomerulus at different age groups have been studied in young (three month-old), adult (twelve month-old) and old (thirty month-old) Fisher strain 344 rats. Pieces of the tissues were taken from renal corticies prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), following by post-fixation with 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3) and embedded within Araldite. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed under a JEM 100CX electron microscope. The mean thickness of glomerular basal lamina and Bowman's capsule were determined by measuring the thinnest portion of basal lamina, and by taking the average of 50 readings from electron micrographs at different ages. The numerical changes of the slit pores were compared based upon the numbers over the length of 10um of glomerular basal lamina. The results were as follow: 1. The thickness of glomerular basal lamina is increased during aging; 140.4 nm in young rats, 270.0 nm in adult ones, and 437.8 nm in old ones. 2. The thickness of basal lamina of parietal cells of Bowman's capsule is 187.5 nm in young rats, 914.0 nm in adult ones, and 2850.0 nm in old ones. 3. The numbers of the slit pores of basal lamina are reduced during aging, 30.3 slit pores/
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