대한미생물학회지 (The Journal of the Korean Society for Microbiology)
- 제22권1호
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- Pages.23-33
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- 1987
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- 0253-3162(pISSN)
렙토스피라용혈소의 검색과 성질
Detection and Property of Leptospiral Hemolysin
- Chang, Woo-Hyun (Department of Microbiology, College of Medicine, Seoul National University) ;
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Kang, Jae-Seung
(Department of Microbiology, College of Medicine, Seoul National University)
- 발행 : 1987.03.31
초록
To detect lepotospiral strains which produce hemolysin and determine the optimal condition for assaying hemolysin, we screened reference strains and observed some property of hemolysin. Hemolysin activity was assayed with cell free culture liquids and erythrocyte suspension. The production of hemolysin by local strains isolated in Korea was assayed and compared with that of reference strains. The hemolysin was produced by 18 strains among 38 reference strains and 3 local strains isolated in Korea. The production of hemolysin began with growth of Leptospira cultured in EMJH medium and reached maximum at stationary phase. The optimum temperature for hemolytic activity was