대한미생물학회지 (The Journal of the Korean Society for Microbiology)
- 제22권2호
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- Pages.163-166
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- 1987
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- 0253-3162(pISSN)
Detection of Antigen-Specific Lymphocytes in Bacillus Calmette Guerin-Infected Mice by a Direct Rosette Assay
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Lee, Hern-Ku
(Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
- Kim, Chong-Shick (Department of Microbiology and Immunology, Chonbuk National University Medical School) ;
-
Ha, Tai-You
(Department of Microbiology and Immunology, Chonbuk National University Medical School)
- 발행 : 1987.06.30
초록
BCG 감염마우스의 비장으로부터 BCG 항원에 대한 수용체를 가진 세포를 BCG 항원으로 감작시킨 면양 적혈구(SRBC)를 이용한 rosette 방법으로 분리하였다. SRBC에 흡착되는 적정 BCG 항원 농도를 알아보기 위하여 tannic acid(1:20,000)으로 전처리한 SRBC에 여러 농도의 BCG 항원으로 작용시킨 결과
A new method for rosette assay is described for the detection of antigen-specific lymphocytes from BCG-infected mice using sheep erythrocytes coated with BCG antigen. The optimal concentration of BCG antigen for preparation of indicator cells and the incubation time of antigen coated erythrocytes-lymphocytes mixture were