대한미생물학회지 (The Journal of the Korean Society for Microbiology)

대한미생물학회 (The Korea Society for Microbiology)
권호 표지

제한효소 DNA 분석법에 의한 국내분리 렙토스피라균의 동정

Restriction Endonuclease DNA Analysis of Leptospiral Field Isolates from Korea

  • 장우현 (서울대학교 의과대학 미생물학교실, 암연구소) ;
  • 김석용 (충북대학교 미생물학교실) ;
  • 서정선 (서울대학교 의과대학 생화학교실, 암연구소)
  • Chang, Woo-Hyun (Department of Microbiology, Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Kim, Suck-Yong (Department of Microbiologyl, College of Medicine, Chungbuk National University) ;
  • Seo, Jeong-Sun (Department of Biochemistry, Cancer Research Institute, College of Medicine, Seoul National University)
  • 발행 : 1987.12.31
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초록

The genomes of leptospiral field isolates from Korea belonging to serogroup Icterohaemorrhagiae (21 strains) and serogroup Canicola (1 strain) were analysed and compared by restriction enzyme analysis with EcoRI and HindIII as digesting enzymes. One isolate belonging to serogroup Canicola showed the same pattern as serovar portlandvere. All 21 isolates belonging to serogroup Icterohaemorrhagiae showed almost same patterns as Leptospira serovar lai from China, But with very slight differences 21 isolates could be classified into 8 subtypes and these grouping seems to reflect the differences in epidemiological niche. And also the geographical data consisted with the grouping into 8 subtypes. According to our results, we concluded that the restriction endonuclease analysis of chromosomal DNA will be an accurate and reliable method to compare and classify pathogenic leptospires.

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