산업기술연구 (Journal of Industrial Technology)

  • 제7권
  • /
  • Pages.59-68
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  • 1987
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  • 1229-9588(pISSN)
  • /
  • 1598-1371(eISSN)
강원대학교 산업기술연구소 (Kangwon National University, Institute of Industrial Technology)
권호 표지

Alkalophilic microorganism이 생산하는 lipase에 관한 연구

Studies on the Lipase Produced by Alkalophilic Microorganism

  • Jeong, Kwang-Seon (Dept. of Fermentation Technology Kangweon National University) ;
  • Ham, Cheol-Joo (Dept. of Fermentation Technology Kangweon National University) ;
  • Shin, Won-Cheol (Dept. of Fermentation Technology Kangweon National University)
  • 발행 : 1987.10.31
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초록

In order to obtain a strain of producing lipase which has resistance against alkaline and detergent, a screening test was carried out. Among 500 strains isolated from soil samples, the strain J-19 was selected for this study. The composition of the optimum medium for the highest lipase production was 2.0% glycerin, 1.0% corn steep liquor, 2.0% yeast extract, 0.1% $MgSO_4$ $7H_2O$, 0.2% $K_2HPO_4$, 1.5% soybean oil and 0.1% LAS(linear alkylbenzene sulfonate) with initial pH value of 10.0 and 3-day cultivation at $25^{\circ}C$. The lipase activity of the strain J-19 under optimal condition was 3.3. units/ml, which was increased about 1.3-fold than that of basal medium.

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