초록
상수리나무(Quercus acutissima) 1년생(年生) 실생묘(實生苗)의 액아(腋芽)를 배양(培養)하여 얻은 기내(器內) shoot를 상(上) 중(中) 하(下)의 세 부분(部分)으로 나누어 다경유도(多莖誘導), 발근(發根) 및 풋트이식시(移植時) 활착율(活着率)을 조사(調査)하였다. 다경유도(多莖誘導)는 하부(下部)>중간부(中間部)>상부(上部)의 순(順)으로 양호(良好)하였으며 하부(下部)를 배양시(培養時) WPM+$1.0g/{\ell}$ BAP+$0.1g/{\ell}$ NAA에서 배양(培養) 4주후 액아(腋芽) 1개(個)에서 평균(平均) 6.2개(個)의 줄기가 유도(誘導)되었다. 발근율(發根率)은 다경유도(多莖誘導)와 반대현상(反對現象)을 보여 상부(上部)>중간부(中間部)>하부순(下部順)으로 양호(良好)하였다. IBA $0.2mg/{\ell}$를 같이 첨가(添加)할 경우 1/2 MS 배지(培地)가 GD배지(培地)보다 발근(發根)이 빠르게 이루어 졌으며 뿌리도 양호(良好)하였고 상부(上部)줄기의 경우(境遇) 1/2 MS 배지(培地)에서 100%의 발근율(發根率)을 보였으며, sucrose 농도별(濃度別) 1/2 MS+IBA $0.2mg/{\ell}$ 배지(培地)에서의 발근시험결과(發根試驗結果)는 sucrose 3~6% 농도(濃度)에서 양호(良好)하였다. 활착율(活着率)에 있어서는 상부(上部)줄기의 발근묘(發根苗)가 양호(良好)하였고(50%) 중(中) 하부(下部)는 생존(生存)하지 못하였다. 활착(活着)된 묘목(苗木)은 조기낙엽현상(早期落葉現象)이 관찰(觀察)되었으나 10개월(個月)까지 생존(生存)하고 있다. 위의 결과(結果)로 상부(上部)는 발근(發根)시켜 pot묘(苗) 생산(生産)에 이용(利用)하고 중(中) 하부(下部) 줄기는 연속증식(連續增殖)에 이용(利用)함이 좋을 것으로 생각된다.
This study was conducted to examine the position effect of axillary buds on shoot multiplication and rooting with 1-year-old seedlings of Quercus acutissima. Shoot multiplication was greatly affected by axillary bud position: Productivity of multiple shoots were decreased in the order of top, mid and basal explants respectively. The best shoot multiplication (mean 6.1 shoots per explant) was obtained on WPM medium containing $1.0mg/{\ell}$ BAP and $0.1mg/{\ell}$ NAA using basal explant after 4-week-culture. Rooting was also greatly influenced by position. Its percentage was increased in the order of top, mid and basal explant respectively. Root initiation was better and more rapid on 1/2MS medium than GD medium. High rooting percentage (100%) was obtained on 1/2MS medium containing $0.2mg/{\ell}$ IBA after 15 days culture. Sucrose concentrations did not effect on rooting. However root development and shoot growth were greatly affected by them. Root was developed shortly on 1-2% levels and shoot growth was getting retarded, whereas both of them did not show significant difference at 3-6% levels. Rotting was decreased on 7-8% levels gradually, but shoot and leaf condition was better than any other concentrations. Survival rate of rooted explants in pot was varied according to the position of explants. Seedlings of top part were survived up to about 50% but most of mid and basal part seedlings did not survive over 4 weeks even in high humidity condition. Seedlings in pots showed normal growth over 10 months but most of them showed the condition of premature leaf shedding.