Aspergillus niger KUF-04가 생산한 Glucose Oxidase의 정제에 관한 연구

Purification of Glucose Oxidase from Aspergillus niger KUF-04

Aspergillus niger KUF-04로부터 얻은 glucose oxidase는 결정화를 포함하여 7단계의 공정을 거쳐 순수하게 정제되었다. 공모양의 결정은 23배 정제된 효소액에서 얻어졌다. 분자량은 HPLC에 의해 210, 000, SDS-polyacrylamide electrophoresis에 의해 110,000을 나타냈으므로 본 효소는 동일한 2개의 subunit로 이루어진 단백질임을 알 수 있었다.

Glucose oxidase from Aspergillus niger KUF-04 was purified homogeneously by the procedure of seven steps including crystallization. The ball-like crystalline enzyme was obtained from the 23-fold purified enzyme solution. The glucose oxidase was found to be composed of two identical subunits and the molecular weight of the enzyme and its subunit were estimated to be about 210, 000 and 110, 000 by HPLC and SDS-acrylamide gel electrophoresis, respectively.