한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제15권6호
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- Pages.381-387
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- 1987
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Bacillus circulans가 생산하는 Superoxide Dismutase에 관한 연구
Study on the Intracellular Superoxide Dismutase Produced by Bacillus circulans
- Lee, Sang-Ok (Department of Microbiology, College of Natural Science, Pusan National University) ;
- Tae-Ho Lee (Department of Microbiology, College of Natural Science, Pusan National University)
- 발행 : 1987.12.01
초록
생체 내에서
Distribution of superoxide dismutase (SOD) which catalyzes the dismutation of superoxide radicals to hydrogen peroxide and oxygen has been examined in various genera of bacteria. SOD was produced by various bacteria independent of genus and species with variation in superoxide dismutase activity of each bacteria. Bacillus circulans which produced relatively large amount of SOD was selected and used to investigate the optimum culture conditions and further studies. The compositions of optimum culture medium for the enzyme production were 1% glucose, 2% polypeptone, 0.l% NaCl, and 0.2mM of methyl viologen and initial pH was 6.0. The highest enzyme production was observed after 20 hours of cultivation at 3