Studies on the Toxic Substsnce of Mussel Mytilus Sp.

담치類의 有毒成分에 관한 硏究

Attempts were made to elucidate the responsible toxin in mussel Mytilus sp. which caused a food pisoning incident in March 1986 in Pusan, Korea. Two persons were dead and 15 persons intoxicated in the incident. The mid-gut glands of the mussel collected were extracted with dichlorlmethane, filtered through a Diaflo ulteafiltration membrane, and then purified by chromatography on Bio-Gel P-2 and Bio-Rex 70. The toxic fractions obtained were analysed by electrophoresis, TLC and ion-pairing reversed phase HPLC analyses. The results showed that the fractions contained GTW$\_$1-4/ as the major component, along with neoSTX, PX$\_$1,2/ as the minor. It was concluded from these results that the causative mussel toxin of the above food poisoning was PSP.

1986年 3月 釜山近郊의 廢船處理場에서 發生한 담치類 Mytilys sp. 에 의한 食中毒의 原因을 밝히기 위해 이 貝類의 毒成分에 관하여 調査하였다. 毒成分은 中腸腺을 酸流出하여, 限外濾過 및 各種 칼럼크로마 토그라피法으로 精製하여, TLC, 電氣泳動 및 HPLC로 分析하였는데, 그 結果 담치毒은 GT $X_{1-4}$를 主成分 으로 하고, neoSTX과 P $X_{1,2}$를 副成分으로 하고 있음을 確認하였다. 따라서 이 食中毒의 原因物質은 痲痺性 貝毒(PSP)인 것이 確認되었으며, 이는 PSP가 國內産 貝類에서 最初로 檢出된 것이다.것이다.