Agrobacterium tumefaciens KU-12 균주에서 분리한 플라스미드 pTi 12의 제한효소 지도

Restriction endonuclease mapping of the plasmid pTi12 from agrobacterium tumefaciens

한국산 A, tumefaciens의 3균주에서 분리한 Ti plasmid의 type과 분자량을 조사한 결과로 이들 모두는 octopine type 이었으며 각 plasmid의 분자량은 pTi12가 44Kb이었고, pTi14는 180Kb이었으며, pTi14는 172Kb이었다. 이중 pTi12를 Smal 및 HindIII로 가수분해한 결과 8개의 Smal digest fragment와 10개의 Hind III digest fragment를 얻었으며 Southern hybridization techniques을 이용하여 잠정적인 physical map을 작성하였다.

Ti plasmids were isolated from three strains of Agrobacterium tumefaciens in Korea and their types and molecular weights were determined. All of these are octopine-type and their molecular weights are 44Kb (pTi 12), 180Kb (pTi 14) and 172Kb (pti 49), respectively. In order to construct physical map of pTi 12, pTi 12 was digested with restriction endonucleases Sma I and Hind III. Sma I degestion of pTi 12 produce 8 fragments and Hind III produced 10 fragments. Physical arrangements of these fragments was determined by Southern hybridization techniques.