Korean Journal of Microbiology (미생물학회지)
- Volume 25 Issue 2
- /
- Pages.148-156
- /
- 1987
- /
- 0440-2413(pISSN)
- /
- 2383-9902(eISSN)
Characterization of laccase from pleurotus ostreatus
Pleurotus ostreatus의 laccase 작용특성
Abstract
Extracellular laccase (E.C. 1.10.3.2) from the culture filtrate of Pleurotus ostreatus was purified by ammonium sulfate precipctation, protamine sulfate precipitation, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel permeation chromatography. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 58,000 and the isoelectric point was 3.75. The optimum temperature for the enzyme was about