Quantitative Analysis of 1,4-Androstadiene-3,17-Dione in Fermentation Broth

발효액중의 1,4-Androstadiene-3,17-Dione의 정량법

Fermentation processes have been employed to produce 17-ketosteroids from sterol with chelator of Fe$^{2+}$ such as $\alpha$, ${\alpha}'$-dipyriyl. To analyze the products of sterol fermentation, we developed a simple TLC method without interference of the chelator $\alpha$, ${\alpha}'$-dipyridyl, using Ag$^+$ band on TLC plate.