Archives of Pharmacal Research
- 제10권4호
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- Pages.212-218
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- 1987
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Agonist-induced Desensitization of Muscarinic Acetylcholine Receptor in Rat Brain
- Lee, Jong-Hwa (Department of Pharmacology and Toxicology University of Maryland) ;
- Esam-E.El-Fakahany (School of Pharmacy 20 N. Pine St.Balitimore)
- 발행 : 1987.12.01
초록
Intact brain cell aggregates were dissociated from adult rat brains without cerebellum using a sieving technique. This proparation was used to elucidate the binding characteristics of agonist to muscarinic acetylcholine receptors (mAchR) in brain. Incubation of cells with carbamylcholine (carbachol) was shown agonist-induced receptor down-regulation depending on the concentration of agonist, not depending on the incubation time. This effect of carbachol was due to a reduction in the maximal binding capacity (
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