Effect of Scoparone on the Hepatic Microsomal UDPglucuronyltransferase Activity in Mice

  • Huh, Keun (Department of Pharmacology, College of Pharmacy, Yeungnam University) ;
  • Lee, Sang-Il (Department of Pharmacology, College of Pharmacy, Yeungnam University) ;
  • Park, Jong-Min (Department of Pharmacology, College of Pharmacy, Yeungnam University)
  • Published : 1987.09.01

Abstract

The effect of scoparone on UDP glucuronyltransfearase in mouse hepatic microsomes was studied. After transment with scoparone, hepatic microsomal UDP glucuronyltransferase activity was increased with dose-dependent manner as compared to control. The V$_max$ value (control = 23.2 n moles/mg protein/min, scoparone = 31.2 n moles/ mg protein /min) without affecting the $K_m$ value (414 $\mu$M) for p-nitrophenol was increased by the scoparone treatment, and the pattern of kinetic studies for UDP-glucuronic acid was also similar to those of p-nitrophenol. Whereas, the hepatic microsomal UDP glucuronyl-transferase was not changed by the addition of scoparone in vitro. The results obtained suggest that the characteristics of increase in the enzyme activity may include induction of enzyme proteins.

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