Applied Biological Chemistry
- 제30권4호
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- Pages.293-299
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- 1987
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
형질전환(形質轉換)에 의한 Ti Plasmid의 Bradyrhizobium japonicum에의 도입(導入)
Introduction of Ti Plasmid into Bradyrhizobium japonicum by Spheroplast Transformation
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Yun, Han-Dae
(Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
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Cho, Moo-Je
(Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
- Park, Chan-Young (Department of Agricultural Chemistry, College of Agriculture, Gyeongsang National University) ;
- Lee, Ke-Ho (College of Agriculture, Seoul National University)
- 발행 : 1987.12.30
초록
Bradyrhizobium japonicum ROKS 26 균주를 glycine및 Iysozyme을 처리하여 spheroplast를 형성시킨 후 Agrobacterium tumefaciens에서 분리한 Ti plasmid를 분리하여 형질전환 시키고 octopine 영양 요구성에 의하여 transformants를 분리하였다. 그 결과 Ti plasmid가 도입된 transformants의 형질전환율은
Bradyrhizobium japonicum spheroplasts were prepared by culturing cells in the presence of glycine, follwed by treatment with lysozyme. The cells were examined by electron microscopy during the formation of spheroplast. Then Ti plasmid from Agrobacterium tumefaciens 15955 was introduced into Bradyrhizobium japonicum by glycine-lysozyme induced spheroplast transformation. After cell wall regeneration, transformants were selected by the ability of utilization of octopine. Transformation were received at a frequency of
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