Studies on Mycotoxin Produced by Aspergillus versicolor

Aspergillus versicolor가 생성(生成)하는 Mycotoxin에 관(關)한 연구(硏究)

The results in vestigated produced fluorescence compounds and mycotoxins, studied stability and toxicity of these compounds which were isolated from varicous moistured rice culture extacts inoculated Aspergillus versicolor IFO 30338, 10 kinds of fluorescence compounds were isolated. Sterigmatocystin was produced $38{\mu}g/kg$ in 16%, $329{\mu}g/kg$ in 25% and $380{\mu}g/kg$ in 35% moistured rice 20 days culture respectively. Aflatoxin $B_1$ was produced $3{\mu}g/kg$ in 25% and $12.6{\mu}g/kg$ in 35% moistured rice 20 days culture. In embryo test of isolated fraction 2, 4 and 6 by T.L.C., $LD_{50}$ of fraction 2 was $40{\mu}g/egg$ and fraction 4,6 was $60{\mu}g/egg$, and these compounds were mostly decomposed and fraction 4 and 6 were partly changed into fraction 2 below pH 2 and above pH 10. fraction 2,4 and 6 were all stable when treated 60 min, at $100^{\circ}C$, but were decomposed $60{\sim}65%$ when treated 60 min at $150^{\circ}C$, $95{\sim}100%$ when treated 10 min at $200^{\circ}C$.

수분 함량을 달리한 쌀에 Aspergillus versicolor를 접종시켜 생성 Mycotoxin및 기타 형광물질을 분석하고 그 안정성과 독성등에 관해 조사한 결과 배양 추출물에서 10증의 형광물질을 분리하였고 Sterigmatocystin은 수분 16배지에서 $38{\mu}g/kg$ 25%에서는 $329{\mu}g/kg$에시는 35%에서는 $380{\mu}g/kg$이 20일 배양시 생성되었으며 Aflatoxin $B_1$은 수분 25%배지에서는 $3{\mu}g/kg$, 35%에서는 $12.6{\mu}g/kg$이 20일 배양시 생성되었다. Embryo Test에서 Fraction 2물질의 $LD_{50}$은 $40{\mu}g/egg$, Fraction 4와 6물질은 $60{\mu}g/egg$이었으며 이들 물질은 pH 2이하, PH 10 이상에서는 거의 분해되었고 Fraction 4와 6물결은 일부가 Fraction 2물질로 변화되었다. 또한 이들 Fraction 2, 4, 6물질은 $100^{\circ}C$에서 60분간 처리로 안정하였으나 $150^{\circ}C$ 60분 처리로 $60{\sim}65%$, $200^{\circ}C$ 10분 처리에서는 $95{\sim}100%$가 분해되었다.