한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제19권6호
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- Pages.506-514
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- 1987
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- 0367-6293(pISSN)
밤생율(生栗)에 함유된 Peroxidase의 정제 및 특성에 관한 연구
Purification and Properties of the Peroxidase in Castanea Semen
- Oh, Suk-Heung (Department of Agricultural Chemistry, Chonbuk National University) ;
- Kim, Yong-Hwi (Department of Agricultural Chemistry, Chonbuk National University) ;
- Lee, Seo-Na (Department of Food Science & Technology, Chonbuk National University)
- 발행 : 1987.12.01
초록
밤(생율(生栗))으로 부터 peroxidase를
Peroxidase was purified to a homogeneous state from Castanea Semen by ammonium sulfate precipitation, DEAE-cellulose column chromatography, gel filtration on sephadex G-100 and HPLC, and the purification fold was 65.3. The molecular weight of the enzyme was estimated to be about 35,000 by HPLC. In properties of the enzyme which was purified up to sephadex G-100 column chromatography, the optimum pH and temperature were 5.0 and
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