Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 19 Issue 5
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- Pages.441-445
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- 1987
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- 0367-6293(pISSN)
Localization of Lipoxygenase in Germinating Soybeans
발아중인 대두콩에서의 Lipoxygenase의 국재(局在)
- Song, Young-Sun (Department of Food and Nutrition, Inje College)
- 송영선 (인제대학 식품영양학과)
- Published : 1987.10.01
Abstract
The subcellular distribution of lipoxygenase in germinating soybean seeds (Glycine max[L.] AmSoy) was investigated by using differential centrifugation and sucrose density gradient fractionation. Most of lipoxygenase -1 and -2/3 activities was present in the supernatant fraction after differential centrifugation of homogenates prepared from three-day-old seedlings; only 1.5% of lipoxygenase activity remained in particulate fractions. The results of a sucrose density gradient fractionation (three-day-old) showed that the lipoxygenase activity coincided with acid phosphatase at the densities of 1.19, 1.23,
발아중인 대두콩에서의 lipoxygenase의 세포내 위치를 차별 원심 분리법과 밀도 구배법을 이용하여 규명하였다. 차별 원심 분리법을 사용하였을때, lipoxygenase활성은 거의 supernatant fraction에서 나타났으며, 1.5%의 활성만이 particulate fractions에서 나타났다. 밀도 구배법에서는 lipoxygenase와 acid phosphatase의 활성이 1.19, 1.23,
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