Abstract
The effect of Ganoderma lucidum extract on Saccharomyces cerevisiae growth and physiology has been investigated. S. cerevisiae was inoculated in Henneberg solution medium into which 0, 0.1, 0.5 or 1.0% extracts of G. lucidum were added respectively and it was fermented at $30^{\circ}C$ for 5 days, respectively. Cell number of S. cerevisiae has increased according to the concentration as in order of distilled water(Dw) extracts 1.0% added>ethanol(Et) extracts 1.0% added>Dw extracts 0.5% added>Et extracts 0.5% added>Dw extracts 0.1% added>Et extracts 0.1% added group compared to control group(extracts 0% added) and in Dw extracts 1.0% added group the number has increased than those of control group after the fermentation of 72 hours. Weights of dried yeast cell have increased in each treated group than those of control group and it increased about 1.7 times in each Dw 1.0%, Et 1.0% group than those of control group after fermentation of 120 hours. The more the extracts of G. lucidum was added, the more alcohol levels increased during fermentation. The rate of carbon dioxide production per G. lucidum extract medium was faster than those of control group as G. lucidum extract was increasingly added.
영지추출물이 효모의 증식과 생리에 미치는 영향을 검토하기 위하여 영지의 용매별 추출물을 0, 0.1, 0.5, 1.0%되게 첨가한 Henneberg 액체배지에 S. cerevisiae를 접종하고, 5일간 발효$(30^{\circ}C)$과정중 균체생산, Alcohol 발효, $CO_2$ 발생 등을 조사하였다. 1. 발효과정중 72시간후 효모 세포수는 증류수추출물(Dw) 1.0% 첨가구>에탄올추출물(Et) 1.0% 첨가구>증류수추출물(Dw) 0.5% 첨가구>에탄올추출물(Et) 0.5%첨가구>증류수추출물(Dw) 0.1% 첨가구>에탄올추출물(Et) 0.1% 첨가구>대조구의 순이고 특히 1.0%구는 대조구보다 5.3배 많았다. 2, 건조균체량은 각 시험구 모두 대조구보다 많았고, Dw 1.0%구와 Et 1.0%구는 120시간 발효 후 대조구에 비해 약 1.7배였다. 3. 알코올 발효과정중 알코올 함량은 영지추출물의 첨가량이 증가함에 따라 많아졌다. 4. 발효과정중 효모의 단위 시간당 $CO_2$ 발생 속도는 영지추출물을 증량할수록 대조구보다 빠르다.